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frt flanked gmr cassette  (Addgene inc)


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    Structured Review

    Addgene inc frt flanked gmr cassette
    Frt Flanked Gmr Cassette, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/frt+flanked+gmr+cassette/miniTn7(Gm)PA1%2F04%2F03-ecfp-a+(Plasmid+%23111619)/pmc10374164__pnas__2302546120__sapp-34-65-74
    Average 93 stars, based on 4 article reviews
    frt flanked gmr cassette - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Expressing:

    Article Title:
    Article Snippet: Mach 1 F-f80lacZDM15 DlacX74 hsdR(rK-, mK+) DrecA1398 endA1 tonA Invitrogen S17-1(lpir) pro, res- hsdR17 (rK- mK+) recA-, attTn7::RP4-2-Tc::MuKm lpir+ (1) JCM736 MC4100 araR DmlaC DpldA (2) .. pBT20 Mariner transposon vector (3) pEXG2 Empty vector used for allelic exchange; GmR (4) pEXG2-PA2800Del mlaA deletion vector; GmR This study pEXG2-PA3211Del PA3211 deletion vector; GmR This study pEXG2PA3238H415A mlaYH415A substitution vector; GmR This study pEXG2-PA3238/9Del mlaYZ deletion vector; GmR This study pEXG2-PA3239Del mlaZ deletion vector; GmR This study pEXG2-PA445XDel mlaBCDEF deletion vector; GmR This study pTNS2 tnsABCD expression vector; AmpR (5) pUC18T-mini-Tn7TGm Vector containing FRT-flanked GmR cassette within miniTn7 element; GmR, AmpR (5) Addgene plasmid #63121 pUC18T-mini-Tn7TPA2800-Gm mlaA expressed from the J23119 promoter within miniTn7 element of pUC18T-mini-Tn7T-Gm; GmR, AmpR This study pUC18T-mini-Tn7TPA3239-Gm mlaZ expressed from the J23119 promoter within miniTn7 element of pUC18T-mini-Tn7T-Gm; GmR, AmpR This study pUC18T-mini-Tn7TPA3238-Gm mlaY expressed from the J23119 promoter within miniTn7 element of pUC18T-mini-Tn7T-Gm; GmR, AmpR This study pUC18T-mini-Tn7TpldA-Gm pldA expressed from the J23119 promoter within miniTn7 element of pUC18T-mini-Tn7T-Gm; GmR, AmpR This study pZS21 Empty E. coli expression vector; KanR (6) pZS21-pldA pldA expressed from the J23119 promoter; KanR This study pZS21-mlaYZ mlaY and mlaZ expressed from the J23119 promoter; KanR This study pZS21-mlaYH415AZ mlaYH415A and mlaZ expressed from the J23119 promoter; KanR This study Table S5: Oligonucleotides used in this study. ..

    Plasmid Preparation:

    Article Title:
    Article Snippet: Mach 1 F-f80lacZDM15 DlacX74 hsdR(rK-, mK+) DrecA1398 endA1 tonA Invitrogen S17-1(lpir) pro, res- hsdR17 (rK- mK+) recA-, attTn7::RP4-2-Tc::MuKm lpir+ (1) JCM736 MC4100 araR DmlaC DpldA (2) .. pBT20 Mariner transposon vector (3) pEXG2 Empty vector used for allelic exchange; GmR (4) pEXG2-PA2800Del mlaA deletion vector; GmR This study pEXG2-PA3211Del PA3211 deletion vector; GmR This study pEXG2PA3238H415A mlaYH415A substitution vector; GmR This study pEXG2-PA3238/9Del mlaYZ deletion vector; GmR This study pEXG2-PA3239Del mlaZ deletion vector; GmR This study pEXG2-PA445XDel mlaBCDEF deletion vector; GmR This study pTNS2 tnsABCD expression vector; AmpR (5) pUC18T-mini-Tn7TGm Vector containing FRT-flanked GmR cassette within miniTn7 element; GmR, AmpR (5) Addgene plasmid #63121 pUC18T-mini-Tn7TPA2800-Gm mlaA expressed from the J23119 promoter within miniTn7 element of pUC18T-mini-Tn7T-Gm; GmR, AmpR This study pUC18T-mini-Tn7TPA3239-Gm mlaZ expressed from the J23119 promoter within miniTn7 element of pUC18T-mini-Tn7T-Gm; GmR, AmpR This study pUC18T-mini-Tn7TPA3238-Gm mlaY expressed from the J23119 promoter within miniTn7 element of pUC18T-mini-Tn7T-Gm; GmR, AmpR This study pUC18T-mini-Tn7TpldA-Gm pldA expressed from the J23119 promoter within miniTn7 element of pUC18T-mini-Tn7T-Gm; GmR, AmpR This study pZS21 Empty E. coli expression vector; KanR (6) pZS21-pldA pldA expressed from the J23119 promoter; KanR This study pZS21-mlaYZ mlaY and mlaZ expressed from the J23119 promoter; KanR This study pZS21-mlaYH415AZ mlaYH415A and mlaZ expressed from the J23119 promoter; KanR This study Table S5: Oligonucleotides used in this study. ..



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    Addgene inc frt flanked gmr cassette
    Frt Flanked Gmr Cassette, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/frt+flanked+gmr+cassette/miniTn7(Gm)PA1%2F04%2F03-ecfp-a+(Plasmid+%23111619)/pmc10374164__pnas__2302546120__sapp-34-65-74
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    frt flanked gmr cassette - by Bioz Stars, 2026-10
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